Composite

Part:BBa_K2928009:Design

Designed by: Lauren Hartz   Group: iGEM19_ASIJ_Tokyo   (2019-10-15)


Modified 0.19 Wheat Albumin (Gly to Glu) expression using TDH3, RBS, and ADH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to select promoters and terminators of appropriate strength. Additionally, we elected not to include a secretion tag in the construct and instead lysed the cells before assessing the activity of the synthesized 0.19.


Source

The majority of the parts in this construct come from previously existing parts on the registry. The sequence for the original 0.19 Wheat albumin (prior to modification) came from the Protein Database.

References